Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Chem. Epub 2012 Oct 18. Colloid Polym. Changes in the Mark-Houwink hydrodynamic volume of chitosan molecules in solutions of different organic acids, at different temperatures and ionic strengths. Biophysical Journal, 87, 4259-4270. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. An official website of the United States government. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. Learn More. Polyelectrolytes as secondary calibration standards for aqueous SEC. An undergraduate physical chemistry experiment. The hydrodynamic 57 radius of the dextranase is 7 WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Granath, K. A. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. 2012;87(6):1728. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Harding, Stephen E. (2005). Saudi Pharm J. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Please enable it to take advantage of the complete set of features! These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Drug Deliv Syst. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Unauthorized use of these marks is strictly prohibited. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. Recent Pat Drug Deliv Formul. National Library of Medicine Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Journal of Applied Polymer Science, 27, 795-810. 1994, 95-0355. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. 113, 81-86. Radiofrequency with microneedling. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Banga AK. The micropores of the human cadaver skin were regenerated according to the time. The effect of branching is best recognized from the molecular weight dependencies of []. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. This situation is mainly the result of using dextans, solvent, additives, etc. Keywords: Morris, G.A. Cite this article. 1984;17(6):11703. osmotic pressure or light scattering). Comparison of single-point and dilution procedures. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Journal Non-Newtonian Fluid Mech., 97, 295-301. Increased epidermal laser fluence through simultaneous ultrasonic microporation. Carbohydrate Polymers 88 1440-1444. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. FITC-dextran solution (600L) was added to the donor chamber. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. Arch Dermatol Res. (2012). After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. Epub 2022 Feb 2. Smith, David R.; Raymonda, John W. (1972). Keywords: FITC dextran; intestinal permeability; transit time. 2005;22(4):5505. Optical properties of dextran in solution and in films. 43-1, 115-147. 2012;27(3):20212. J Appl Toxicol. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Nordmeier, Eckliard (1993). (1998). ; Dutt, N.V.K. 2010;4(1):117. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. By using this website, you agree to our There is a deviation from linearity after 580kDa [69, 70]. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Thus, it is a common practice to express the frictional coefficient of rigid structures as. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Front Microbiol. Biomacromolecules 8, 2464-2475. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. J Pharm Sci. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. ; Patel, T.R. Article Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. (1990). Dynamics of a Polymer Solution in a Rigid Matriz. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. Journal of Physical Chemistry, 58(11), 953-957. Differences were considered statistically significant at p <0.05. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. European Biophysical Journal, 36, 85-94. Skin barrier disruption by acetone: observations in a hairless mouse skin model. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. The hydrodynamic radius of 2-NBDG glucose was too small to measure. (2004). Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. ; Weisz, J. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. WebTry out PMC Labs and tell us what you think. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. (2003). Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Advances in Carbohydrate Analysis, 1, 63-144. volume24, Articlenumber:22 (2020) With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Weissella: An Emerging Bacterium with Promising Health Benefits. European Polymer Journal, 37, 619-622. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Unable to load your collection due to an error, Unable to load your delegates due to an error. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Transdermal drug delivery is an attractive alternative that addresses the limitations of oral and parenteral routes of drug administration; it enables controlled release and long-term systemic drug delivery through the skin, avoids hepatic first-pass effect, avoids gastrointestinal drug degradation, reduces discomfort and trauma resulting from hypodermic injections, and prevents safety hazards associated with dangerous medical waste from needles [1, 2]. Provided by the Springer Nature SharedIt content-sharing initiative. Carbohydrate Polymers 88 373-381. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Solution properties of branched dextran. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Nat Biotechnol. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. These results are in agreement with a previous study which suggested that the microporation using radiofrequency causes disruption of the SC, enabling the percutaneous permeation of the FITC-dextran [21]. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Naik A, Kalia YN, Guy RH. Determination of intrinsic viscosities of macromolecules and nanoparticles. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. ; Rjumtsev, E.I. Pure Appl. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). After 8h, the micropore became more distorted from the previous circle, suggesting that the micropores were closing. Ahn, G.Y., Eo, HS., Kim, D. et al. Table 2 compares the properties of nanoparticles coated with one or other polymer. 2013;43(4):287303. Webdextran hydrodynamic radius. De Belder, A. N. (2001). The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. The skin poration was performed using the microporation device (LG electronics, Korea). Kasaai, M. R.; Arul, J.; Charlet, G. (2000). The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. 2003;89(2):31120. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Would you like email updates of new search results? Advances in chemistry series Washington, DC: American Chemical Society., pp. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Polymer molecular weight distribution. statement and Corp. (Seoul, Korea). European Polymer Journal, 36, 2143-2146. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Progress in Polymer Science, 30, 38-70. Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. (2000). Transdermal drug delivery: overcoming the skins barrier function. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". (1937). The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Eremeeva, T. E.; Bykova, T. O. The human and pig skin permeability of FITC-dextran (M.W. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Solvent effects on polysaccharide conformation. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Stenekes, R.J.H. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Google Scholar. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Viscotek GmbH, Europe. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. Ioan, C.E. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo.