Accessed April 2011. Flow Cytometry: Principles and Clinical Applications in Hematology Clinical Chemistry 46:8(B) 12211229 [On-line information]. Anaplastic lymphoma kinase protein was detected in about 33% (3/9) of ALCLs examined by flow cytometric immunophenotyping (FCI); expression was validated by immunohistochemical analysis. Sometimes pieces of the abnormal myeloma protein are filtered through the kidney into the urine. Available online at https://www.cancer.gov/cancertopics/factsheet/detection/laboratory-tests. FOIA These abnormalities were related to immunophenotypic markers as This study prospectively analysed the relationships between immunophenotypic and cytogenetic features of blast cells in 432 acute non-lymphoblastic leukemias (ANLL) at presentation. Even normal aging can make cells appear abnormal. Available online at https://www.mayomedicallaboratories.com/test-catalog/Overview/3287. Acute Lymphoblastic Leukemia. As the number of abnormal cells increases in the bone marrow, they may crowd out and inhibit the production of normal white blood cells, red blood cells, and platelets, and eventually abnormal cells may also be released into the blood. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). National Library of Medicine No significant immunophenotypic abnormality was detected by flow cytometry. Jiang NG, Jin YM, Niu Q, Zeng TT, Su J, Zhu HL. MDS is distinguished from other disease processes by a pattern of multiple myeloid immunophenotypic abnormalities (3-6). ( 2015). Careers. Abstract. Normal granulocytes show sequential progression from promyelocytes . Leuk Res. Clinical Laboratory Medicine. 1. Accordingly, a score of 0.5, 1 or 2 was given when the value obtained for . This category is to be used to record an episode of elevated blood pressure in a patient in whom no formal diagnosis of hypertension has been made, or as an isolated incidental finding. In these cases, LSC analysis is a methodology of choice because of its low sample requirements. Kanwar, V. et. MayoClinic [On-line information]. Flow cytometry immunophenotyping may be useful in helping to diagnose, classify, treat and determine prognosis of these blood cell cancers. The results from your immunophenotyping are compared to the pattern of antigens for normal cells as well as to patterns that are associated with abnormal cells (e.g., cells present with leukemias and lymphomas). (2008 December 1). Trisomy 12 is the second most frequent aberration detected by fluorescence in situ hybridization at the time of diagnosis (10-25%), and it confers an . Application of these criteria to a series of nearly 500 cases of lymphoma indicated that over 90% of B-lineage and about 80% of T-lineage neoplasms manifested immunophenotypic abnormalities that could distinguish them from benign, reactive lymphoid processes. The present results further confirm that IGH@ rearrangement is not a rare genomic abnormality in B-CLL, and also show both that t(14;19)(q32;q13.2) is the most common cytogenetic change involving IGH@ rearrangement detected by FISH in B-CLL and that IGH@ rearrangement is correlated with CD38 expression. Cheriyedath, Susha. Flow cytometric immunophenotyping for hematologic neoplasms. Jevremovic D, Olteanu H: Flow cytometry applications in the diagnosis of T/NK-cell lymphoproliferative disorders. In the present study, we describe both quantitative and qualitative immunophenotypic abnormalities involving BM B-cells in MDS patients. Sometimes, however, the cancer cells adapt to evade the therapy by not expressing anymore an antigen that they expressed earlier, which might have been targeted by a monoclonal antibody or other therapy, like CAR T-cells. Specimens will be initially triaged to determine which, if any, of the immunophenotyping panels should be performed. A total of 192 Chinese patients with acute myeloid leukemia (AML) were immunophenotyped by flow cytometry using a panel of monoclonal antibodies. This test is not appropriate for and cannot support diagnosis of sarcoidosis, hypersensitivity pneumonitis, interstitial lung diseases, or differentiating between pulmonary tuberculosis and sarcoidosis (requests for CD4/CD8 ratios); specimens sent for these purposes will be rejected. Accessed January 2020. Am J Clin Pathol. The site is secure. The t(14;19)(q32;q13) involving the IGH@ and BCL3 loci is an infrequent cytogenetic abnormality detected in B-cell malignancies. This abnormal protein is known by several different names, including monoclonal immunoglobulin, monoclonal protein (M protein), M spike, or paraprotein. Verbal Irony In Romeo And Juliet Act 2. http://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=341450, http://www.nature.com/leu/journal/v20/n7/full/2404242a.html, http://www.bloodjournal.org/content/96/3/870?sso-checked=true. Bethesda, MD 20894, Web Policies PMC ( 19952014). Ann Hematol. Before No flow cytometric abnormalities were detected in CD4-positive T-cells from 10 control patients without lymphoproliferative disorders. Viability 7AAD: 99%. In our case report, a middle-aged male . Initial evaluation of . MeSH The results may also be used to predict how aggressive the cancer will be and/or whether it will respond to certain treatment. An interpretation of the immunophenotypic findings and correlation with the morphologic features will be provided by a hematopathologist for every case. What is Immunophenotyping?. American Cancer Society. Accessed April 2011. The above negative findings can be attributed to low leukemia burden in the BMA. Morphological, immunophenotypic, and genetic features of chronic A pathologist, often one specializing in the study of blood diseases and/or blood cell cancers (a hematopathologist), will consider the results from the complete blood count (CBC), differential, blood smear, bone marrow findings, and flow cytometry immunophenotyping as well as other tests in order to provide a diagnostic interpretation. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Careers. Please enable it to take advantage of the complete set of features! Accessed April 2011. Frequent CD7 antigen loss in aggressive natural killer-cell leukemia: a useful diagnostic marker. This site needs JavaScript to work properly. official website and that any information you provide is encrypted [Flow cytometric analysis of surface phenotypes in B-cell non-Hodgkin's lymphoma]. Merck Manual for Healthcare Professionals [On-line information]. This site needs JavaScript to work properly. Novel Biological Insights and New Developments in Management of Burkitt Lymphoma and High-Grade B-Cell Lymphoma. 2004 Mar;121(3):373-383. doi: 10.1309/3A32-DTVM-H640-M2QA, 7. We describe the clinicopathologic, cytogenetic, and molecular genetic characteristics of 14 cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with t(14;19)(q32;q13). These newer treatments may have reduced side effects compared to conventional chemotherapy (newer targeted therapies are usually added to traditional chemotherapy). In addition, reflex testing may occur to fully characterize a disease state or clarify any abnormalities from the screening test. Accessed January 2020. By continuing to browse this site you agree to our use of cookies. Available online at https://www.questdiagnostics.com/hcp/intguide/jsp/showintguidepage.jsp?fn=TG_Lymphoid_Neoplasms.htm. 1990 Oct;81(10):629-34. (Updated 2014 March 23). If . Unauthorized use of these marks is strictly prohibited. Immunophenotypic analysis of non-Hodgkin's lymphomas. Medeiros BC, Kohrt HE, Arber DA, Bangs CD, Cherry AM, Majeti R, Kogel KE, Azar CA, Patel S, Alizadeh AA. Available online at https://www.clinchem.org/cgi/content/full/46/8/1221. Among B-lineage populations the following features were associated with malignant histology: 1) light-chain-restricted B lineage, 2) light chain -B lineage, 3) Leu-1+ B lineage, 4) L60+ B lineage, 5) 41H+, Ki-67+ B lineage, 6) loss of pan-B antigens, and 7) LFA-1-B lineage. Compilation of the top interviews, articles, and news in the last year. An official website of the United States government. Flow cytometric analysis of the peripheral blood shows no immunophenotypic evidence for an abnormal B cell or T- cell population, and no circulating blasts. The immunophenotype of different immature, myeloid and B-cell lineage sharing sensitive information, make sure youre on a federal official website and that any information you provide is encrypted Li Y, Wei J, Mao X, Gao Q, Liu L, Cheng P, Liu L, Zhang X, Zhang K, Wang J, Zhu L, Zhou J, Zhang Y, Meng L, Sun H, Li D, Huang M, Huang W, Deng J, Zhang D. PLoS One. Available online at https://arupconsult.com/content/acute-lymphoblastic-leukemia. Type and frequency of immunophenotypic alterations detected on PB platelets from MDS patients (n = 44) versus normal control subjects (n=20). Leukemia/Lymphoma Immunophenotyping by Flow Cytometry. Body fluid samples are obtained through collection of the fluid in a container or by inserting a needle into the body cavity and aspirating a portion of the fluid with a syringe. Br J Haematol. The objective of the present study was to assess whether a Compass database-guided analysis can be used to . Leukemia & Lymphoma Society [On-line information]. (Revised 2012). Immunophenotypic, cytogenetic and clinical features of 192 AML patients (2018 March 12). Torpy, J. Your questions will be answered by a laboratory scientist as part of a voluntary service provided by one of our partners, American Society for Clinical Laboratory Science. Please enable it to take advantage of the complete set of features! Flow cytometry is generally used to determine cell lineage in leukemia and lymphoma. Specimen Stability Information: Ambient/Refrigerated < or =96 hours, Slides: If possible, include 5 to 10 unstained bone marrow aspirate smears labeled with two unique identifiers. In this article, News-Medical talks to Sartorius about biosensing and bioprocessing in gene therapy, There is increasing evidence of T cell dysfunction in B cell chronic lymphocytic leukaemia (B-CLL) which may contribute to the aetiology and progress of the disease. 1. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen: Specimens will be initially triaged to determine which, if any, of. Am J Clin Pathol. Cheriyedath, Susha. ARUP Consult. Acute myeloid leukemias (AMLs) are hematologic malignancies with varied molecular and immunophenotypic profiles, making them difficult to diagnose and classify. Bookshelf Several studies have identified a relationship between AML prognosis and antigens such as CD7, CD9, CD11b, CD13, CD14, CD15, CD33, CD34, and CD56, though some other studies report conflicting results. How Is Childhood Leukemia Diagnosed? Clinical features, laboratory findings, morphologic, cytogenetic features, and Epstein-Barr virus status were important factors for diagnosing aggressive NK cell leukemia. [Importance of cytogenetics in the study of acute non-lymphoblastic leukemias]. Accessed January 2020. Unable to load your collection due to an error, Unable to load your delegates due to an error. Because of this, immunophenotyping results will be different by reflecting the current population of WBCs that would be present in an individual in remission. Immunophenotypic criteria for the diagnosis of non-Hodgkin's - PubMed Non-Hodgkin's lymphoma presenting as a primary cardiac lymphoma (PCL) is extremely unusual. Blood Tests. Available online at https://www.mayoclinic.com/health/chronic-lymphocytic-leukemia/DS00565. Diagnosis of malignant lymphoma - An overview. (2012 February 17). Objectives: To report aberrant myeloblasts detected by flow cytometry immunophenotypic studies in an asymptomatic patient with familial platelet disorder with propensity to myeloid malignancy, a rare autosomal dominant disease caused by germline heterozygous mutations in Runt-related transcription factor 1. Tel19p/19q used to detect copy number abnormalities of chromosome 19, reveal a hybridization pattern within normal limits in 200 analyzed nuclei. Epub 2018 May 7. Szary syndrome with multiple immunophenotypic aberrancies in tumor cells. This case suggested that chromosomal alterations may precede morphological, flow cytometric and clinical changes and accelerate progression of the disease. Lymphoid Neoplasms Laboratory Support of Diagnosis and Management Test Guide. Mayo Clinic Staff (2010 November 24). Immunophenotyping is widely used to identify and classify AML. Recenti Prog Med. More info. Federal government websites often end in .gov or .mil. For bone marrow testing, if cytogenetic tests are desired along with this test request, an additional specimen should be submitted. MeSH terms Chromosome Aberrations and transmitted securely. Examples of signs and symptoms of a blood cell cancer include: Testing may also be ordered after you have been treated for leukemia or lymphoma. It is important that the specimen be obtained, processed, and transported according to instructions for the other test. If no abnormalities are detected by the initial panel, no further flow cytometric assessment will be performed unless otherwise indicated by specific features of the clinical presentation or prior laboratory results. Leuk Lymphoma. 2009 Dec;29(6):491-6. doi: 10.3343/kjlm.2009.29.6.491. American Society for Clinical Pathology; 2007; Betters DM: Use of flow cytometry in clinical practice. This technique involves immunostaining of smears of fluids from body cavities or aspirates of tissues. Flow cytometry immunophenotyping is used primarily to help diagnose and classify blood cell cancers (leukemias and lymphomas) and to help guide their treatment. It can detect normal cells as well as abnormal cells whose pattern of markers are typically seen with specific types of leukemia and lymphoma. Disclaimer. no immunophenotypic abnormalities detected - salongmaria.se Discussion. Anders PM, Montgomery ND, Montgomery SA, Bhatt AP, Dittmer DP, Damania B. J Clin Invest. Mcclellan Oscillator Website, Cancers (Basel). Multivariate analysis identified CD34 and CD9 expression as independently predictive of the presence of at least one cytogenetic abnormality (P < 10(-4) and P < 0.03, respectively). A cell count should be determined and submitted with the specimen. PMC Fonatsch C, Gudat H, Lengfelder E, Wandt H, Silling-Engelhardt G, Ludwig WD, Thiel E, Freund M, Bodenstein H, Schwieder G, et al. (2018 October 17, Revised). (2022, December 30). Application of these criteria to a series of nearly 500 cases of lymphoma indicated that over 90% of B-lineage and about 80% of T-lineage neoplasms manifested immunophenotypic abnormalities that could distinguish them from benign, reactive lymphoid processes. gayle telfer stevens husband Order Supplement. (Blood cells normally mature in the bone marrow and are released into circulation when they are mature or nearly mature.) [Co-occurrence of t(8;21)(q22;q22) and t(9;22)(q34;q11) in a - PubMed 2013 Jul;346(1):56-63. doi: 10.1097/MAJ.0b013e3182764b59. Interestingly, some of the other antigens present might suggest a specific genetic subtype of B-lymphoblastic leukemia, which also might have a certain prognosis. 1. LCMS - Overview: Leukemia/Lymphoma Immunophenotyping, Flow Cytometry 1. Bronchoalveolar lavage specimens submitted for evaluation for leukemia or lymphoma are appropriate to send for this test. The markers (antigens) that are present on the cells as detected by flow cytometry immunophenotyping will help characterize the cells present. doi: 10.1371/journal.pone.0158827. MedlinePlus Medical Encyclopedia [On-line information]. Specimen must arrive within 96 hours of collection. TdT and PAX5 were performed in five of the seven patients with ABLB detected by FC. A positive correlation was found between CD34+ and CD34 B-cell precursors (r . 2023 TESTING.COM. Trisomy 12 is the second most frequent aberration detected by fluorescence in situ hybridization at the time of diagnosis (10-25%), and it confers an intermediate prognostic risk, with a median time to . It may be used in follow up to a complete blood count (CBC) and WBC differential that show an increased number of lymphocytes or the presence of immature blood cells or other abnormal cell counts. Jevremovic D, Dronca RS, Morice WG, et al: CD5+ B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Chronic lymphocytic leukemia is an extremely heterogeneous disease and prognostic factors such as chromosomal abnormalities are important predictors of time to first treatment and survival. Siba El Hussein, Keyur P. Patel, Hong Fang, Beenu Thakral, Sanam Loghavi, Rashmi Kanagal-Shamanna, Sergej Konoplev, Elias J. Jabbour, L. J. Jeffrey Medeiros, Joseph D. Khoury Furthermore, these findings can also be seen Incidence of peripheral lymphadenopathy, hepatic abnormalities, splenic abnormalities, and abdominal lymphadenopathy was not significantly different among immunophenotypic groups. MDS is distinguished from other disease processes by a pattern of multiple myeloid immunophenotypic abnormalities (3-6). Map Of Southern Maine And New Hampshire, Stay up to date with the latest news and information from Testing.com by subscribing to our newsletter. Would you like email updates of new search results? Tissue flow cytometry immunophenotyping in the diagnosis and Web: mayocliniclabs.com: Email: mcl@mayo.edu: Telephone: 800-533-1710: International: +1 855-379-3115: Values are valid only on day of printing For the individual abnormalities detected for each of the 27 immunophenotypic variables analyzed, a score was defined. Immunophenotypic analysis is an established tool in the diagnosis and classification of many hematolymphoid disorders; however, the role of flow cytometry (FC) in detecting bone marrow involvement during the staging of non-Hodgkin lymphoma (NHL) has yet to be defined. In agreement with previous studies, no immunophenotypic features (other than monocytic differentiation) predicted the presence of an 11q23 rearrangement. The type of sample to be tested is up to your healthcare practitioner and must be representative of your cancer. Cytometry B Clin Cytom. 3. eCollection 2022. Because of the heterogeneity and commonly associated cytogenetic abnormalities AML-MRC has no specific immunophenotypic profile. An original cytospin preparation (preferably unstained) must be included with the spinal fluid specimen so correlative morphologic evaluation can occur. Available online at https://www.cancer.org/cancer/leukemia-in-children/detection-diagnosis-staging/how-diagnosed.html. The prognostic value of immunophenotyping in AML is controversial [ 3]. If abnormal cells are present in the bloodstream, a blood sample is often used for flow cytometry immunophenotyping as it is easy to obtain and less invasive than other collection methods. American Cancer Society: Tests for Acute Lymphocytic Leukemia (ALL), CD19, CD20, CD22, CD79a, immunoglobulin light chains (kappa or lambda), CD2, CD3, CD5, CD7, and either CD4 or CD8, Megakaryocytic differentiation; Platelets, Red blood cell (erythroid) differentiation, To predict how aggressive the cancer will be, To predict whether the cancer will respond to certain treatment, To help determine whether treatment of leukemia or lymphoma has been successful, To determine whether the disease remains despite treatment (residual disease) or has come back after successful treatment (recurrent disease), Shortness of breath during normal physical activity, Enlarged lymph nodes, spleen, liver, kidneys, and/or testicles. A stable aberrant immunophenotype characterizes nearly all cases of It is concluded that immunophenotypic analysis of lymphoproliferative lesions is sufficiently sensitive and specific to confirm the histologic diagnosis of lymphoma in the vast majority of cases seen in clinical practice. The screening panel will be charged based on the number of markers tested (FIRST for first marker, ADD1 for each additional marker). CD38 expression is not detected (<10%) No evidence of p53 (17p13) 4. Miao Y, Zhang J, Chen Q, Xing L, Qiu T, Zhu H, Wang L, Fan L, Xu W, Li J. Second, unusual expression of surface antigens in ANKL cells was a prominent feature. Bookshelf Atypical or abnormal cells can demonstrate . Hu X, Yang Y, Chen L, Wan Y, Sheng L, Bao Y, Zheng M. Am J Transl Res. Leukemias and lymphomas are caused by an abnormal white blood cell that begins to divide uncontrollably, making numerous copies of itself (clones). NCI CPTC Antibody Characterization Program. An additional complicating factor is antigenic shift, 13 , 20 although the number of cases in which immunophenotypically aberrant blasts convert to an . Third, the clonality of ANKL cells could be identified using antibodies against CD158a/h, CD158b, or CD158e. In this interview, we speak to Ceri Wiggins, a Director at AstraZeneca, about the many applications of CRISPR and its role in discovering new COPD therapies. no immunophenotypic abnormalities detected. Rarely, no overt immunophenotypic abnormality will be present at diagnosis, and in these cases, the sensitivity of flow cytometric evaluation for minimal residual disease may be greatly reduced. National Library of Medicine A positive correlation was found between CD34+ and CD34 B-cell precursors (r . Do not aliquot. The most common patterns of post-relapse FISH dissimilarity were loss of previously detected hyperdiploidy, seen in three (33.3%) cases, and gain of 1q21 in three (33.3%) cases. Category filter: Show All (140)Most Common (2)Technology (21)Government & Military (34)Science & Medicine (22)Business (30)Organizations (68)Slang / Jargon (8) Acronym Definition NSA National Security Agency (US government) NSA Naval Support Activity NSA National Speakers Association NSA No Strings Attached NSA Naczelny Sad Administracyjny (Polish . Abnormal karyotypes were detected in 76 out of 125 (60.8%). In this interview, AZoM speaks to Rohan Thakur, the President of Life Science Mass Spectrometry at Bruker, about what the opportunities of the market are and how Bruker is planning on rising to the challenge. Abnormal immunophenotype profiles are usually present in: The following summarizes markers that are often expressed in certain types of cells: The following summarizes markers that suggest certain types of cell differentiation: T-lymphocyte subset analysis based on CD3, CD4 and CD8 expression is performed separately to monitor people with HIV/AIDS, for example. Genomic and immunophenotypic landscape of aggressive NK-cell leukemia. It depends. Accessed April 2011. American Cancer Society. For spinal fluid specimens: spinal fluid cell and differential counts are required. J Adv Pract Oncol. Immunophenotypic and antigen receptor gene rearrangement analysis in T cell neoplasia. An ASCUS pap smear result is considered to be mildly abnormal. It is also suggested to have prognostic significance [ 2]. Accessed January 2020. Accessed April 2011. Flow cytometric immunophenotyping of peripheral blood, bone marrow, and body fluids is performed using the following antibodies: Triage Panel: CD3, CD10, CD16, CD19, CD34, CD45 and kappa and lambda light chains, -B-cell Panel: CD5, CD11c, CD19, CD20, CD22, CD23, CD38, CD45, CD103, CD200 and kappa and lambda light chains, -T-cell Panel: CD2, CD3, CD4, CD5, CD7, CD8, CD45, TRBC1, and gamma/delta, -Killer-cell immunoglobulin-like receptor (KIR) Panel: CD3, CD8, CD16, CD56, CD57, CD94, CD158a, CD158b, CD158e (p70), and NKG2a, -Acute Panel: CD2, CD7, CD13, CD15, CD16, CD33, CD34, CD36, CD38, CD45, CD56, CD64, CD117, and HLA-DR, -B-cell ALL, minimal residual disease (MRD) panel: CD10, CD19, CD20, CD22, CD24, CD34, CD38, CD45, CD58, and CD66c, -Myeloperoxidase (MPO)/terminal deoxynucleotidyl transferase (TdT) (MPO/TdT) Panel: cytoplasmic CD3, CD13, cytoplasmic CD22, CD34, CD45, cytoplasmic CD79a, nuclear TdT, and cytoplasmic MPO, -Plasma Cell Panel: CD19, CD38, CD45, CD138, and cytoplasmic kappa and lambda light chains, -Mast Cell Panel: CD2, CD25, CD69, CD117. Conclusion: Only 5 similar cases have been described previously. 4th ed. Unable to load your collection due to an error, Unable to load your delegates due to an error. . These may be the first indication of a possible blood cell cancer. Clinical review on features and cytogenetic patterns in adult acute myeloid leukemia with lymphoid markers. 1985 Aug 29;313(9):539-44 Pp 244-247. Grave Encounters What Happened To Kenny, Flowcytometric Immunophenotypic Characterization of Acute Myeloid SI Abnormal Reports. Leuk Lymphoma. 1989 May;91(5):579-83. doi: 10.1093/ajcp/91.5.579. Now, if an adult has a small number of mature B cells but also has a large number of immature B cells which are positive for CD19 (remember, CD19 is a B-cell marker) and also positive for both CD34 and CD20 (which identifies those cells are both immature and abnormal), then the personhasan immature B-cell leukemia known as B-lymphoblastic leukemia. Additional FISH or molecular testing may be recommended by the Mayo pathologist to facilitate diagnosis. B-cell leukemia/lymphoma panel. HHS Vulnerability Disclosure, Help Abnormal spacing of fully erupted tooth or teeth NOS; Displacement of fully erupted tooth or teeth NOS; Transposition of fully erupted . No abnormalities were detected for the other phenotypic markers analyzed, including 7.1 ( Table 2 ). According to the immunophenotype, MBL is labeled as chronic lymphocytic leukemia (CLL)-like (75% of cases), atypical CLL, and CD5-negative. Leukemic myeloblasts expressed many leukocyte differentiation antigens, thus reflecting association with myeloid lineage and maturation level. MeSH There is a dim Kappa expression and dim CD20 expression. (FNA09-1171; 9/30/09): No monotypic B cell population, phenotypically abnormal T cell population, or blast cell population detected. Accessed December 2014. -, N Engl J Med. If additional testing is required, it will be added per the algorithm to fully characterize a disease state with a charge per unique antibody tested. 2008 December 1; 112(12): 43844399. Available online at https://www.nlm.nih.gov/medlineplus/ency/article/003518.htm. This technique also helps identify or confirm the cell of origin in non-hematopoietic neoplasia. (PDF) Immunophenotypic Analysis of Anaplastic Large Cell - ResearchGate Accessed December 2014. Mayo Clinic, Mayo Medical Laboratory [On-line information]. By junio 4, 2022 masonry pilaster details junio 4, 2022 masonry pilaster details This technique helps identify the lineage of cells using antibodies that detect markers or antigens on the cells, hence the immuno- prefix. To help diagnose and classify a leukemia or lymphoma; to help guide treatment; to aid in determining prognosis; to detect and evaluate leukemia or lymphoma cells that remain after treatment or at disease relapse, When you have signs and symptoms that a health care practitioner thinks may be due to leukemia or lymphoma; to help classify the type of leukemia or lymphoma, identify treatment options, and predict the likely course of the disease; to evaluate whether treatment has been effective or detect disease that remains or comes back after treatment (relapse or recurrence).